Fish Kills
Can a fish die-off happen to you?
By Bob Lusk
I have seen several cases of toxicity due to aflatoxin from moldy feed. If you have moldy feed, don't use it.
http://www.bassresource.com/fish_biology/fish-kill.htmlPathology of Afloatoxin
High-level aflatoxin exposure produces an acute hepatic necrosis, resulting later in cirrhosis, and/or carcinoma of the liver. Acute hepatic failure is manifested by hemorrhage, edema, alteration in digestion, and absorption and/or metabolism of nutrients and mental changes and/or coma.
No animal species is immune to the acute toxic effects of aflatoxins including humans; however, humans have an extraordinarily high tolerance for aflatoxin exposure and rarely succumb to acute aflatoxicosis.
http://en.wikipedia.org/wiki/AflatoxinI think this is the cause of your losses. Low DO kills fish outright and there would have been signs of distress before this- surface gasping; stressed fish usually get diseases before they end up dying; the fact that you fed moldy fish feed probably poisioned them. The mold could have been caused by mice urine as mentioned above but most often, it is caused by having feed in a high moisture area. Feed of any kind should be used up in less than six months and kept cool but dry. Even the protein in the feed, which is usually a fish meal or fish oil source, can go rancid and again cause poisioning.
Check the livers of your fish and you will have your answer. If you don't know how, there may be a university or college that will do a toxicology for free. Call around- a lot of people don't know that colleges and universities do this type of thing but it is good practice for students. Also call your county extension office, often times they know places that may cost nothing or very little.
I would suggest cleaning out your growbeds, anywhere the feed settled and change out all of your water. I don't know how much toxin is required to kill fish or to stress them, which may lead to other problems in the future. Maybe the above references can help with that question as well.
Sorry for your loss. I think you are the first one here, that I know of, that has had this problem.